soluble cd40 ligand (scd40l)  (PeproTech)

Journal: Clinical and Applied Thrombosis/Hemostasis

Article Title: Risk of Atherosclerosis Due to HMGB1-dependent Platelet-derived Microparticles in Patients with Type 2 Diabetes Mellitus

doi: 10.1177/10760296241302082

Figure Lengend Snippet: Multivariate Analysis of HMGB1 in Patients with T2DM.

Article Snippet: ELISA kits for soluble vascular cell adhesion molecule (sVCAM-1), plasminogen activator inhibitor (PAI-1), interleukin-6 (IL-6), soluble E-selectin (sE-selectin), regulated on activation, normal T cell expressed and secreted (RANTES), soluble P-selectin (sP-selectin), soluble CD40 ligand (sCD40L) and monocyte-chemoattractant protein (MCP)-1 were purchased from BioSource International, Inc. (Camarillo, California, USA).

Techniques:

Journal: Brain, Behavior, & Immunity - Health

Article Title: Peripheral inflammation and depressed mood independently predict neurocognitive worsening over 12 years

doi: 10.1016/j.bbih.2022.100437

Figure Lengend Snippet: Correlations of baseline depression severity and Factor 1 with change in global cognition. Shaded areas represent 95% confidence bands for the regression fit. sqrt (BDI-II), square root Beck Depression Inventory-II. CRP, C-reactive protein. IL-6, interleukin-6. sCD40L, soluble CD40 ligand. sAPP, serum amyloid precursor protein.

Article Snippet: Markers of inflammation included C-reactive protein [CRP] (Laboratory Corporation of America, San Diego, CA), interleukin-6 [IL-6], soluble tumor necrosis factor receptor type II [sTNFR-II], and monocyte chemoattractant protein type 1 [MCP-1] (Meso Scale Discovery, Rockville, Maryland), D-dimer (BioMedica, Windsor, Nova Scotia, Canada), soluble CD14 [sCD14] (R&D, Minneapolis, Minnesota), neopterin (ALPCO, Salem, New Hampshire), and soluble CD40 ligand [sCD40L] (Millipore Sigma, Burlington, Massachusetts).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiplatelet Activity of Tetramethylpyrazine via Regulation of the P2Y12 Receptor Downstream Signaling Pathway

doi: 10.1155/2022/7941039

Figure Lengend Snippet: Effect of SQ22536 and LY294002 on the release of sCD40L and IL-1 β . Results are expressed as mean ± SD. n = 6. & P < 0.05 vs. resting; ∗ P < 0.05 vs. control; # P < 0.05 vs. model; + P < 0.05 vs. TMP; ▲ P < 0.05 vs. SQ22536; △ P < 0.05 vs. LY294002.

Article Snippet: The IL-1 β and soluble CD40 ligand (sCD40L) ELISA kits were obtained from Elabscience Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Control

Journal: The Journal of allergy and clinical immunology

Article Title: Autonomous role of Wiskott-Aldrich syndrome platelet deficiency in inducing autoimmunity and inflammation

doi: 10.1016/j.jaci.2017.12.1000

Figure Lengend Snippet: Interaction between platelets (PLTs) and B cells mediated by CD40L. A, Platelet counts from age-matched WT, WKO, Rag1−/−, and WKO- Rag1−/− mice are shown. B, Evaluation of IgG antiplatelet autoantibodies. ELISA plates were coated with total protein lysates from WT or Was−/− platelets and incubated with sera according to the grid presented (n = 23). C, The graph represents the percentage of mice positive for the anti-double-stranded DNA autoantibodies. D, Percentage of CD40L+ platelets was evaluated by using flow cytometry before (n = 10) and after (n = 12) stimulation with thrombin (1 U/mL; left); sCD40L was measured in PPP from unwashed platelets isolated from WT (n = 16), WKO (n = 16), and CoWas (n = 5) mice (right). E, Resting B cells (CD43− cells) isolated from the spleens of WT, WKO, and CoWas mice were kept in culture for 72 hours with PPP and analyzed by using flow cytometry for CD69 expression as an activation marker. In Fig 6, A, each dot represents a different mouse, and means ± SDs are reported. In Fig 6, B, D, and E, the mean ± SD reported is the result of 2 (Fig 6, B) and 4 (Fig 6, D) independent experiments. Statistical analysis was performed with 1-way ANOVA and the Bonferroni postcorrection test (Fig 6, A, B, and D, left panel). In Fig 6, D, sCD40L levels (rightpanel) are analyzed with the Mann-Whitney test, as well as Fig 6, D. Fig 6, C, has been analyzed statistically with the χ2test. *P <.05, **P < .005, ***P < .001, and ****P < .0001.

Article Snippet: Anti–double-stranded DNA antibodies were evaluated by means of ELISA, as described by Bosticardo et al. 27 The presence of anti-PLT autoAbs was assessed, as described by Brigida et al. 28 ELISA kits to evaluate concentrations of murine soluble CD62P (sCD62P; eBioscience, San Diego, Calif) and plasma soluble CD40 ligand (sCD40L; eBioscience, San Diego, Calif) were used according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Flow Cytometry, Isolation, Expressing, Activation Assay, Marker, MANN-WHITNEY

Journal: The Journal of allergy and clinical immunology

Article Title: Autonomous role of Wiskott-Aldrich syndrome platelet deficiency in inducing autoimmunity and inflammation

doi: 10.1016/j.jaci.2017.12.1000

Figure Lengend Snippet: Activation profile of human platelets. A and B, Activation profile of human platelets expressed as the MFI ratio of activation markers and CD61 MFI (Fig 7, A, for CD62P and Fig 7, B, for PAC1). We assigned to the unstimulated adult HD (HD a) a value of 100, and we normalized the patients accordingly. In Fig 7, A, we analyzed 4 patients (3 with WAS and 1 with XLT) and 7 HDs; in Fig 7, B, we analyzed 3 patients (2 with WAS and 1 with XLT) and 5 HDs. C, The level of sCD40L in the plasma of patients with WAS/XLT was analyzed by means of ELISA. Patients with WAS/XLT were divided according to the presence or absence of autoantibodies or other clinical signs related to autoimmunity (see Table E3). Pediatric HDs (HD p) were tested as control subjects. Fig 7, A and B, show means analyzed with the Mann-Whitney test. Fig 7, C, reports means ± SDs and shows analysis with 1-way ANOVA and the Bonferroni postcorrection test. *P < .05 and **P < .005.

Article Snippet: Anti–double-stranded DNA antibodies were evaluated by means of ELISA, as described by Bosticardo et al. 27 The presence of anti-PLT autoAbs was assessed, as described by Brigida et al. 28 ELISA kits to evaluate concentrations of murine soluble CD62P (sCD62P; eBioscience, San Diego, Calif) and plasma soluble CD40 ligand (sCD40L; eBioscience, San Diego, Calif) were used according to the manufacturer’s instructions.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY